Fig. 5. BACE1 specific peptide treatment of SY5Y cells reduces the amount of Aβ40 and Aβ42 production. SY5Y cells grown to confluence were treated with concentrations of BACE1 specific peptide at 1nm, 10nM and 100nm in media. Naturally grown cells were tested with inhibitor and analysed by ELISA for (a) Aβ40 and (b) Aβ42. Testing for oxidative stress on SY5Y cells and potential for amyloid control by BACE1 specific peptide treatment at concentrations of 1nM, 10nM and 100nM. H₂O₂ induced stress on treated cells were tested by ELISA for the production of (c) Aβ40 and (d) Aβ42 and calculated for the percentage reduction as a result of the treatment. All values are reported as individual data points (n=3) and bars represent mean percentage reduction. Statistical significance on raw data where treatments are compared to the negative control is determined by unpaired Student's t-test (p<0.05 *, p<0.01 **, p<0.001 ***, p<0.0001 ****).